{"id":2803,"date":"2017-07-24T16:12:05","date_gmt":"2017-07-24T08:12:05","guid":{"rendered":"http:\/\/chundubio.com\/?p=2803"},"modified":"2017-07-24T16:12:05","modified_gmt":"2017-07-24T08:12:05","slug":"qpcr%e5%b8%b8%e8%a7%81%e9%97%ae%e9%a2%98%e5%8f%8a%e5%88%86%e6%9e%90%e8%a7%a3%e5%86%b3%e6%96%b9%e6%b3%95","status":"publish","type":"post","link":"http:\/\/chundubio.com\/?p=2803","title":{"rendered":"qPCR\u5e38\u89c1\u95ee\u9898\u53ca\u5206\u6790\u89e3\u51b3\u65b9\u6cd5"},"content":{"rendered":"\n\n\n
\u505a\u8fc7PCR\u7684\u7ae5\u978b\u4eec\u90fd\u77e5\u9053\u8981\u60f3\u5b8c\u5168hold\u8be5\u5b9e\u9a8c\u3001\u6210\u4e3a\u771f\u6b63\u7684PCR\u8fbe\u4eba\uff0c\u5e76\u975e\u6613\u4e8b\u3002\u5c31qPCR\u800c\u8a00\uff0c\u4f3c\u4e4e\u5176\u4e2d\u7684\u6bcf\u4e00\u73af\u8282\u90fd\u53ef\u4ee5\u8ba9\u6574\u4e2a\u5b9e\u9a8c\u6302\u6389\uff0c\u4e5f\u56e0\u6b64\u6210\u4e3a\u4e86\u8d44\u6df1\u79d1\u7814\u72d7\u4eec\u5fc3\u4e2d\u7684\u75db\u3002\u4eca\u5929\u5c0f\u7f16\u5c31\u6574\u7406\u4e86\u4e00\u4e9b\u6709\u5173qPCR\u5e38\u89c1\u7684\u95ee\u9898\u53ca\u5206\u6790\u89e3\u51b3\u529e\u6cd5\u5206\u4eab\u7ed9\u5927\u5bb6\u3002<\/p>\n

\u90a3\u4ec0\u4e48\u662fqPCR\u5462\uff1f
\nqPCR\u7684\u82f1\u6587\u5168\u540d\u662fReal-time Quantitative PCR Detecting System\u3002\u5373\u5b9e\u65f6\u8367\u5149\u5b9a\u91cf\u6838\u9178\u6269\u589e\u68c0\u6d4b\u7cfb\u7edf\uff0c\u4e5f\u53eb\u5b9e\u65f6\u5b9a\u91cf\u57fa\u56e0\u6269\u589e\u8367\u5149\u68c0\u6d4b\u7cfb\u7edf\uff0c\u7b80\u79f0qPCR\u3002<\/p>\n

\u7b80\u5355\u6765\u8bf4\uff0cqPCR\u5c31\u662f\u5229\u7528\u8367\u5149\u4fe1\u53f7\u7684\u53d8\u5316\u5b9e\u65f6\u76d1\u6d4bPCR\u6269\u589e\u53cd\u5e94\u4e2d\u6bcf\u4e00\u4e2a\u5faa\u73af\u6269\u589e\u4ea7\u7269\u91cf\u7684\u53d8\u5316\uff0c\u901a\u8fc7Ct\u503c\u548c\u6807\u51c6\u66f2\u7ebf\u7684\u5206\u6790\u5bf9\u8d77\u59cb\u6a21\u677f\u8fdb\u884c\u5b9a\u91cf\u5206\u6790\u3002\u800c\u5e38\u89c4\u7684PCR\u662f\u65e0\u6cd5\u5bf9\u8d77\u59cb\u6a21\u677f\u51c6\u786e\u5b9a\u91cf\uff0c\u65e0\u6cd5\u5bf9\u6269\u589e\u53cd\u5e94\u5b9e\u65f6\u76d1\u6d4b\u3002<\/p>\n

qPCR\u7684\u7591\u96be\u6742\u95ee<\/strong><\/p>\n

Q1 \u5982\u4f55\u63d0\u9ad8RT-PCR\u53cd\u5e94\u7684\u7075\u654f\u5ea6\u4e0e\u7279\u5f02\u6027\uff1f<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. \u9996\u5148\u786e\u5b9a\u6a21\u677fRNA\u5b8c\u6574\u6027\u597d\uff0c\u65e0DNA\u6c61\u67d3\u3002<\/p>\n

2. RNA\u6a21\u677f\u4e2d\u4e0d\u5e94\u542b\u6709\u6269\u589e\u53cd\u5e94\u6291\u5236\u5242\u3002<\/p>\n

3. \u4e3a\u4e86\u9632\u6b62\u6a21\u677f\u964d\u89e3\uff0c\u5728\u53cd\u5e94\u4f53\u7cfb\u4e2d\u52a0\u5165RNase\u6291\u5236\u5242RNasin\u3002<\/p>\n

4. \u4f7f\u7528\u9002\u91cf\u7684\u6a21\u677fRNA\uff0c\u6a21\u677f\u91cf\u592a\u591a\u4f1a\u964d\u4f4e\u7279\u5f02\u6027\uff0c\u592a\u5c11\u4f1a\u5bfc\u81f4\u6269\u589e\u4e0d\u51fa\u6761\u5e26\u6216\u6761\u5e26\u592a\u5f31\u3002<\/p>\n

5. \u82e5\u6a21\u677f\u4e2d\u6709\u4e8c\u7ea7\u7ed3\u6784\uff0c\u53ef\u901a\u8fc7\u63d0\u9ad8\u9006\u8f6c\u5f55\u53cd\u5e94\u6e29\u5ea6\u6765\u63d0\u9ad8\u6269\u589e\u6548\u679c\u3002<\/p>\n

6. \u8bbe\u8ba1\u5f15\u7269\u65f6\uff0c\u907f\u514d\u5728\u5f15\u72693\u2019\u7aef\u542b\u6709\u4e92\u8865\u5e8f\u5217\uff0c\u907f\u514d\u5f62\u6210\u5185\u90e8\u53d1\u5361\u7ed3\u6784\u3002<\/p>\n

Q2 \u907f\u514dRNA\u964d\u89e3\u7684\u65b9\u6cd5\u6709\u54ea\u4e9b\uff1f<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. \u5728\u7528\u6765\u9a8c\u8bc1\u5b8c\u6574\u6027\u4e4b\u524d\u5148\u5728\u53d8\u6027\u80f6\u4e0a\u5206\u6790RNA\u3002<\/p>\n

2. \u4f7f\u7528\u826f\u597d\u65e0\u6c61\u67d3\u6280\u672f\u5206\u79bbRNA\u3002<\/p>\n

3. \u5c06\u7ec4\u7ec7\u4ece\u52a8\u7269\u4f53\u53d6\u51fa\u540e\u7acb\u523b\u63d0\u53d6RNA\uff0c\u5e76\u5c06\u63d0\u53d6\u597d\u7684RNA\u53cd\u8f6c\u5f55\u4e3acDNA\u8fdb\u884c\u4f4e\u6e29\u4fdd\u5b58\u3002<\/p>\n

Q3 RNA\u4e2d\u542b\u6709\u9006\u8f6c\u5f55\u6291\u5236\u5242\u65f6\uff0c\u600e\u4e48\u5904\u7406\uff1f<\/strong><\/p>\n

Answer\uff1a<\/p>\n

\u9006\u8f6c\u5f55\u6291\u5236\u5242\u5305\u62ec\uff1aSDS\u3001EDTA\u3001\u7518\u6cb9\u3001\u7126\u78f7\u9178\u94a0\u3001\u4e9a\u7cbe\u80fa\u548c\u80cd\u76d0\uff0c\u53ef\u5c06\u5bf9\u7167RNA\u540c\u6837\u54c1\u6df7\u5408\uff0c\u540c\u5bf9\u7167RNA\u53cd\u5e94\u6bd4\u8f83\u4ea7\u91cf\u4ee5\u68c0\u6d4bRNA\u6291\u5236\u5242\uff1b\u82e5\u5bf9\u7167RNA\u4e0e\u6837\u54c1\u6df7\u5408\u540e\u4ea7\u91cf\u964d\u4f4e\uff0c\u5219\u8bf4\u660e\u6837\u54c1\u4e2d\u5b58\u5728\u9006\u8f6c\u5f55\u6291\u5236\u5242\uff0c\u53ef\u752870%\uff08v\/v\uff09\u4e59\u9187\u5bf9RNA\u6c89\u6dc0\u8fdb\u884c\u6e05\u6d17\uff0c\u4ee5\u9664\u53bb\u6291\u5236\u5242\u3002<\/p>\n

Q4 \u5982\u4f55\u51cf\u5c11RNA\u6a21\u677f\u4e2d\u7684\u4e8c\u7ea7\u7ed3\u6784\uff1f<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. \u5c06RNA\u548c\u5f15\u7269\u5728\u4e0d\u542b\u76d0\u53ca\u7f13\u51b2\u6db2\u6761\u4ef6\u4e0b\u53d8\u6027\u3001\u9000\u706b\uff1b\u63d0\u9ad8\u9006\u8f6c\u5f55\u53cd\u5e94\u6e29\u5ea6\u3002<\/p>\n

2. \u6e29\u5ea6\u8d85\u8fc760\u2103\u65f6\uff0c\u4e0d\u80fd\u4f7f\u7528oligo\uff08dT\uff09\u5f15\u7269\uff0c\u9009\u62e9\u4e00\u4e2a\u5728\u53cd\u5e94\u6e29\u5ea6\u53ef\u4ee5\u9000\u706b\u7684GSP\u3002<\/p>\n

3. RT-PCR\u4ea7\u7269\u7684\u957f\u5ea6\u8d85\u8fc71kb\u65f6\uff0c\u53cd\u5e94\u6e29\u5ea6\u9700\u4fdd\u6301\u572865\u2103\u3002<\/p>\n

Q5 RNA\u4e2d\u6709DNA\u6c61\u67d3\u7684\u5904\u7406\u65b9\u5f0f<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. \u82e5\u662f\u57fa\u56e0\u7ec4DNA\u7684\u6c61\u67d3\uff0c\u53ef\u4f7f\u7528DNaseI\u5904\u7406RNA\uff1b \u540c\u65f6\u8bbe\u7f6e\u6ca1\u6709\u9006\u8f6c\u5f55\u7684\u5bf9\u7167\u7ec4\u53cd\u5e94\u68c0\u6d4bDNA\u6c61\u67d3\u3002<\/p>\n

2. \u82e5\u662f\u53d7\u5230\u5916\u6e90DNA\u7684\u6c61\u67d3\uff0c\u53ef\u4f7f\u7528\u6297\u6c14\u96fe\u5242\u548cUDG\u9176\u3002<\/p>\n

Q6 qPCR\u63a2\u9488\u8bbe\u8ba1\u7684\u4e00\u822c\u539f\u5219\u6709\u54ea\u4e9b\uff1f<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. \u6269\u589e\u7247\u6bb5\u7684\u957f\u5ea6\u4e0d\u5e94\u592a\u5927\uff0c\u4e00\u822c\u5c0f\u4e8e300bp\u3002<\/p>\n

2. \u63a2\u9488\u4e0d\u80fd\u548c\u4efb\u4e00\u5f15\u7269\u4e92\u8865\uff0c\u4e14\u5176\u957f\u5ea6\u5728\u4fdd\u8bc1\u7279\u5f02\u6027\u7684\u524d\u63d0\u4e0b\u5c3d\u53ef\u80fd\u7684\u77ed\uff0c\u957f\u5ea6\u4e0d\u8981\u8d85\u8fc730bp\u3002<\/p>\n

3. \u63a2\u9488\u7684Tm\u503c\u81f3\u5c11\u6bd4\u5f15\u7269\u7684Tm\u503c\u9ad85\u5ea6\u3002<\/p>\n

4. \u63a2\u9488\u5982\u7528\u4e8e\u68c0\u6d4b\u591a\u6001\u4f4d\u70b9\uff0c\u591a\u6001\u4f4d\u70b9\u5e94\u5c3d\u53ef\u80fd\u9760\u8fd1\u63a2\u9488\u4e2d\u90e8\u3002<\/p>\n

5. \u63a2\u94885\u2019\u7aef\u4e0d\u80fd\u662f\u78b1\u57faG\uff0cG\u5bf9\u8367\u5149\u57fa\u56e2\u6709\u731d\u706d\u4f5c\u7528\u3002<\/p>\n

Q7\u65e0\u53cd\u8f6c\u5f55\u9176\u60c5\u51b5\u4e0b\uff0c\u5bf9\u7167RNA\u4ecd\u6709\u6269\u589e\u7ed3\u679c<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. \u4f53\u5916\u8f6c\u5f55\u65f6\u4e0d\u53ef\u80fd\u5c06\u6240\u6709DNA\u6a21\u677f\u6d88\u9664\uff0c\u56e0\u800c\u5bf9\u7167\u7ec4\u4f1a\u542b\u6709\u75d5\u91cf\u7684DNA\u3002\u5efa\u8bae\u53ef\u5c06\u7b2c\u4e00\u94fecDNA\u7a00\u91ca1:10\u30011:100\u30011:1000\u500d\u4ee5\u6d88\u9664DNA\u6c61\u67d3\u9020\u6210\u7684\u5f71\u54cd\u3002<\/p>\n

2. \u53ef\u80fd\u662f\u5f15\u7269\u4e8c\u805a\u4f53\u7684\u6761\u5e26\u3002<\/p>\n

Q8 \u6269\u589e\u4ea7\u7269\u6ede\u7559\u5728\u52a0\u6837\u5b54\u4e2d<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. \u53ef\u80fd\u662f\u7531\u4e8e\u6a21\u677f\u91cf\u8fc7\u9ad8\u800c\u5bfc\u81f4PCR\u7ed3\u679c\u4ea7\u751f\u4e86\u9ad8\u5206\u5b50\u91cf\u7684DNA\u80f6\u72b6\u7269\u3002\u5efa\u8bae\u5c06\u7b2c\u4e00\u94fecDNA\u6d53\u5ea6\u7a00\u91ca\u81f3100\u500d\u518d\u8fdb\u884c\u4e8c\u6b21\u6269\u589e\u3002<\/p>\n

2. \u5728\u4e8c\u6b21PCR\u65f6\uff0c\u4f7f\u7528\u7684\u9000\u706b\u6e29\u5ea6\u5982\u679c\u6bd4\u5f15\u7269\u7684Tm\u503c\u4f4e5\u2103\uff0c\u53ef\u5c06\u9000\u706b\u6e29\u5ea6\u9002\u5f53\u589e\u9ad8\u6216\u8fdb\u884c\u70ed\u542f\u52a8\u4ee5\u63d0\u9ad8\u7279\u5f02\u6027\u3002<\/p>\n

Q9 \u65e0Ct\u4fe1\u53f7\u51fa\u73b0<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. \u53cd\u5e94\u5faa\u73af\u53c2\u6570\u4e0d\u591f\uff0c\u4e00\u822c\u8981\u572835\u4e2a\u5faa\u73af\u4ee5\u4e0a\uff0c\u4f46\u662f\u8fc7\u591a\u7684\u5faa\u73af\u6b21\u6570\u53ef\u589e\u52a0\u80cc\u666f\u503c\u3002<\/p>\n

2. \u68c0\u6d4b\u8367\u5149\u4fe1\u53f7\u7684\u6b65\u9aa4\u6709\u8bef\u3002SYBRgreen\u6cd5\uff08SG\u6cd5\uff09\u91c7\u7528\u7684\u662f72\u2103\u5ef6\u4f38\u65f6\u91c7\u96c6\u8367\u5149\u4fe1\u53f7\uff0ctaqman\u6cd5\u5219\u662f\u5728\u9000\u706b\u7ed3\u675f\u6216\u5ef6\u4f38\u7ed3\u675f\u65f6\u8fdb\u884c\u4fe1\u53f7\u91c7\u96c6\u3002<\/p>\n

3. \u5f15\u7269\u6216\u63a2\u9488\u964d\u89e3\uff0c\u53ef\u7528PAGE\u7535\u6cf3\u68c0\u6d4b\u5176\u5b8c\u6574\u6027\uff0c\u82e5\u662f\u7535\u6cf3\u6761\u5e26\u5448\u5f25\u6563\u72b6\uff0c\u53ef\u8003\u8651\u91cd\u65b0\u5408\u6210\u5f15\u7269\u6216\u63a2\u9488\u3002<\/p>\n

4. \u6a21\u677f\u4e0d\u8db3\u6216\u964d\u89e3\uff0c\u5219\u53ef\u4ee5\u91cd\u65b0\u63d0\u53d6\u6838\u9178\u6a21\u677f\u3002<\/p>\n

Q10 Ct\u503c\u51fa\u73b0\u8fc7\u665a\uff08Ct>38\uff09<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. \u6269\u589e\u6548\u7387\u4f4e\uff0c\u53cd\u5e94\u6761\u4ef6\u4e0d\u591f\u4f18\u5316\uff0c\u964d\u4f4e\u9000\u706b\u6e29\u5ea6\uff0c\u589e\u52a0\u9541\u79bb\u5b50\u6d53\u5ea6\u3002<\/p>\n

2. \u53cd\u5e94\u6210\u5206\u964d\u89e3\u6216\u52a0\u6837\u91cf\u4e0d\u8db3\u3002<\/p>\n

3. PCR\u4ea7\u7269\u8fc7\u957f\uff0c\u4e00\u822c\u91c7\u752880-150bp\u3002<\/p>\n

Q11 \u6807\u51c6\u66f2\u7ebf\u7ebf\u6027\u5173\u7cfb\u4e0d\u4f73<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. \u52a0\u6837\u5b58\u5728\u8bef\u5dee\uff0c\u662f\u6837\u54c1\u6d53\u5ea6\u4e0d\u6210\u68af\u5ea6\u3002<\/p>\n

2. \u6807\u51c6\u54c1\u51fa\u73b0\u964d\u89e3\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002<\/p>\n

3. \u5f15\u7269\u6216\u8005\u63a2\u9488\u8bbe\u8ba1\u4e0d\u4f73\u3002<\/p>\n

4. \u6a21\u677f\u4e2d\u5b58\u5728\u6291\u5236\u7269\u6216\u6a21\u677f\u6d53\u5ea6\u8fc7\u9ad8\u3002<\/p>\n

Q12 \u6eb6\u89e3\u66f2\u7ebf\u5b58\u5728\u591a\u4e2a\u4e3b\u5cf0<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. \u5f15\u7269\u8bbe\u8ba1\u4e0d\u591f\u4f18\u5316\u3002<\/p>\n

2. \u5f15\u7269\u6d53\u5ea6\u4e0d\u4f73\uff0c\u4e0a\u4e0b\u6e38\u5f15\u7269\u6d53\u5ea6\u6bd4\u4f8b\u4e0d\u4e00\u81f4\u3002<\/p>\n

3. \u9541\u79bb\u5b50\u6d53\u5ea6\u8fc7\u9ad8\u3002<\/p>\n

4. \u6a21\u677f\u57fa\u56e0\u7ec4\u7684\u6c61\u67d3\u3002<\/p>\n

Q13 \u540c\u4e00\u6837\u54c1\u4e2d\uff0c\u5176\u4e2d\u67d0\u4e00\u4e2a\u8367\u5149\u4fe1\u53f7\u7279\u522b\u5f3a<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. \u8bd5\u5242\u914d\u5236\u65f6\u53cd\u5e94\u6db2\u6ca1\u6709\u5b8c\u5168\u6eb6\u5316\uff0c\u5bfc\u81f4\u63a2\u9488\u91cf\u5728\u4e00\u7ba1\u589e\u591a\u3002<\/p>\n

2. \u8bd5\u5242\u914d\u5236\u65f6\u6ca1\u6709\u5145\u5206\u6df7\u5300\u81f4\u5404\u7ba1\u4e2d\u5404\u6210\u5206\u7684\u91cf\u4e0d\u540c\u3002<\/p>\n

3. PCR\u4eea\u70ed\u69fd\u88ab\u8367\u5149\u7269\u8d28\u6c61\u67d3\uff0c\u9700\u8981\u6e05\u9664\u70ed\u69fd\u4e2d\u7684\u6c61\u67d3\u3002<\/p>\n

Q14 \u6269\u589e\u66f2\u7ebf\u6709\u4e00\u5411\u4e0a\u6216\u5411\u4e0b\u7684\u5c16\u5cf0\uff1f<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. \u53cd\u5e94\u8fc7\u7a0b\u4e2d\u7535\u538b\u4e0d\u7a33\u5b9a\u3002<\/p>\n

2. \u53ef\u80fd\u572820\u4e2a\u5faa\u73af\u5de6\u53f3\u65f6\uff0c\u4eea\u5668\u6709\u505c\u4e0b\u6216\u4eea\u5668\u6709\u5f00\u76d6\uff0c\u4f7f\u5149\u7ebf\u7a81\u7136\u589e\u5f3a\u3002<\/p>\n

3. \u5982\u679c\u5c16\u5cf0\u5411\u4e0b\uff0c\u53ef\u80fd\u662f\u7531\u5364\u7d20\u706f\u8001\u5316\u6240\u81f4\uff0c\u8fd9\u65f6\u5e94\u66f4\u6362\u3002<\/p>\n

Q15 \u90e8\u5206\u6837\u672c\u6269\u589e\u6548\u7387\u8fc7\u4f4e\uff1f<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. \u63d0\u53d6\u6db2\u6b8b\u7559\uff0c\u4e00\u5b9a\u7a0b\u5ea6\u6291\u5236\u4e86PCR\u53cd\u5e94\u3002<\/p>\n

2. \u53cd\u5e94\u6db2\u672a\u4e25\u683c\u53d6\u91cf\u6df7\u5300\u6216\u5206\u88c5\u4e0d\u5747\u5300\u3002<\/p>\n

3. \u8bd5\u5242\u5931\u6548\u3002<\/p>\n

Q16 \u9634\u6027\u5bf9\u7167\u6216\u7a7a\u767d\u5bf9\u7167\u7fd8\u5c3e<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. \u6a21\u677f\u63d0\u53d6\u73af\u5883\u6216\u64cd\u4f5c\u8fc7\u7a0b\u6709\u6c61\u67d3\u3002<\/p>\n

2. \u914d\u6db2\u8fc7\u7a0b\u5b58\u5728\u6c61\u67d3\u3002<\/p>\n

Q17 \u76f4\u7ebf\u578b\u6269\u589e\u66f2\u7ebf<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. \u63a2\u9488\u90e8\u5206\u964d\u89e3\uff1a\u4e00\u822c\u7a00\u91ca\u7684\u63a2\u9488\u53ef\u57284\u00b0C\u4fdd\u5b58\u81f3\u5c113\u4e2a\u6708\uff0c\u63a2\u9488\u7684\u53cd\u590d\u51bb\u878d\u6216\u5bfc\u81f4\u964d\u89e3\uff1b\u6216\u8005\u63a2\u9488\u5728\u5149\u7ebf\u4e0b\u66b4\u9732\u65f6\u95f4\u592a\u957f\u4e86\u3002<\/p>\n

2. \u53cd\u5e94\u6db2\u4e2d\u6709PCR\u6291\u5236\u7269\u3002<\/p>\n

Q18 \u6ca1\u6709\u6269\u589e\u66f2\u7ebf<\/strong><\/p>\n

Answer\uff1a<\/p>\n

1. PCR\u53c2\u6570\u8bbe\u7f6e\u9519\u8bef\uff0c\u5728\u8bbe\u8ba1\u5faa\u73af\u53c2\u6570\u65f6\u5c06\u8367\u5149\u4fe1\u53f7\u8bfb\u53d6\u65f6\u95f4\u8bbe\u5728\u53cd\u5e94\u7684\u7b2c\u4e00\u6b65\uff0c\u5373stage1\u9636\u6bb5\u3002<\/p>\n

2. \u7535\u8111\u8bbe\u5b9a\u4e86\u81ea\u52a8\u4f11\u7720\u3002<\/p>\n

Q19 \u57fa\u7ebf\u4e0b\u6ed1<\/strong><\/p>\n

Answer\uff1a<\/p>\n

\u57fa\u7ebf\u9009\u53d6\u8303\u56f4\u4e0d\u5bf9\uff0c\u53ef\u8bd5\u7740\u5c06\u57fa\u7ebf\u8303\u56f4\u6539\u5927\u4e00\u4e9b\uff0c\u8fd9\u4e00\u95ee\u9898\u5e38\u56e0\u8bd5\u5242\u8d28\u91cf\u6240\u81f4\u3002<\/p>\n

Q20 \u6269\u589e\u66f2\u7ebf\u65ad\u88c2<\/strong><\/p>\n

Answer\uff1a<\/p>\n

\u57fa\u7ebf\u9009\u53d6\u8303\u56f4\u4e0d\u5bf9\uff0c\u57fa\u7ebf\u7ec8\u70b9\u5927\u4e8eCt\u503c\uff0c\u8fd9\u901a\u5e38\u662f\u7531\u4e8e\u6a21\u677fDNA\u6d53\u5ea6\u8fc7\u9ad8\u6240\u81f4\uff0c\u56e0Ct\u503c<15\uff0c\u800c\u57fa\u7ebf\u8303\u56f4\u4ecd\u53d63-15\uff0c\u5176\u4e2d\u5305\u542b\u90e8\u5206\u6269\u589e\u4fe1\u53f7\uff0c\u5bfc\u81f4\u6807\u51c6\u5dee\u504f\u5927\uff0c\u9608\u503c\u8fc7\u9ad8\uff0c\u89e3\u51b3\u529e\u6cd5\uff1a\u51cf\u5c11\u57fa\u7ebf\u7ec8\u70b9\u81f3Ct\u524d4\u4e2a\u5faa\u73af\uff0c\u91cd\u65b0\u5206\u6790\u6570\u636e\u3002<\/p>\n

Q21 \u6837\u54c1\u6d53\u5ea6\u8de8\u5ea6\u8fc7\u5927<\/strong><\/p>\n

Answer\uff1a<\/p>\n

\u6837\u54c1\u6d53\u5ea6\u8fc7\u9ad8\uff0c\u5bfc\u81f4\u9633\u6027\u6837\u54c1\u6269\u589e\u66f2\u7ebf\u5728\u540e\u9762\u5faa\u73af\u4e2d\u5448\u4e00\u5411\u4e0b\u7684\u76f4\u7ebf\uff0c\u5176\u89e3\u51b3\u65b9\u6cd5\u540c\u201c\u6269\u589e\u66f2\u7ebf\u65ad\u88c2\u201d\u3002<\/p>\n

Q22 \u5c71\u5761\u5f62\u66f2\u7ebf<\/strong><\/p>\n

Answer\uff1a<\/p>\n

\u5e38\u56e0\u53cd\u5e94\u7ba1\u5c01\u53e3\u4e0d\u4e25\uff0c\u81f3\u53cd\u5e94\u6db2\u84b8\u53d1\u6240\u81f4\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

\u505a\u8fc7PCR\u7684\u7ae5\u978b\u4eec\u90fd\u77e5\u9053\u8981\u60f3\u5b8c\u5168hold\u8be5\u5b9e\u9a8c\u3001\u6210\u4e3a\u771f\u6b63\u7684PCR\u8fbe\u4eba\uff0c\u5e76\u975e\u6613\u4e8b\u3002\u5c31qPCR\u800c\u8a00\uff0c\u4f3c\u4e4e\u5176\u4e2d\u7684\u6bcf\u4e00 […]<\/p>\n","protected":false},"author":1,"featured_media":73,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"http:\/\/chundubio.com\/index.php?rest_route=\/wp\/v2\/posts\/2803"}],"collection":[{"href":"http:\/\/chundubio.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/chundubio.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/chundubio.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/chundubio.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2803"}],"version-history":[{"count":1,"href":"http:\/\/chundubio.com\/index.php?rest_route=\/wp\/v2\/posts\/2803\/revisions"}],"predecessor-version":[{"id":2804,"href":"http:\/\/chundubio.com\/index.php?rest_route=\/wp\/v2\/posts\/2803\/revisions\/2804"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/chundubio.com\/index.php?rest_route=\/wp\/v2\/media\/73"}],"wp:attachment":[{"href":"http:\/\/chundubio.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2803"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/chundubio.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2803"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/chundubio.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2803"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}