{"id":1750,"date":"2017-07-17T08:30:29","date_gmt":"2017-07-17T00:30:29","guid":{"rendered":"http:\/\/chundubio.com\/?p=1750"},"modified":"2017-07-17T08:30:29","modified_gmt":"2017-07-17T00:30:29","slug":"%e4%b8%b4%e5%ba%8a%e5%8f%8a%e7%a7%91%e7%a0%94%e6%a0%b7%e5%93%81-elisa-%e6%a3%80%e6%b5%8b%e6%9c%8d%e5%8a%a1","status":"publish","type":"post","link":"http:\/\/chundubio.com\/?p=1750","title":{"rendered":"\u4e34\u5e8a\u53ca\u79d1\u7814\u6837\u54c1 ELISA \u68c0\u6d4b\u670d\u52a1"},"content":{"rendered":"

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\n\u25c7<\/strong>\u00a0<\/strong>\u6ce8\u610f\u4e8b\u9879\uff1a<\/strong>\u00a0<\/strong>
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\n\u25c7<\/strong>\u00a0<\/strong>\u6db2\u4f53\u7c7b\u6807\u672c\uff1a<\/strong>\u00a0<\/strong>
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\n\u25c7<\/strong>\u00a0<\/strong>\u8840\u6e05\uff1a<\/strong>\u00a0<\/strong>
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\n\u25c7<\/strong>\u00a0<\/strong>\u8840\u6d46\uff1a<\/strong>\u00a0<\/strong>
\n\u5e94\u6839\u636e\u8bd5\u5242\u76d2\u7684\u8981\u6c42\u9009\u62e9 EDTA \u3001\u67e0\u6aac\u9178\u94a0\u6216\u809d\u7d20\u4f5c\u4e3a\u6297\u51dd\u5242\uff0c\u52a0\u5165 10 \uff05\uff08 v\/v \uff09\u6297\u51dd\u5242 ( 0.1M \u67e0\u6aac\u9178\u94a0\u6216 1% heparin \u6216 2.0%EDTA.Na2) \u6df7\u5408 10-20 \u5206\u949f\u540e\uff0c\u79bb\u5fc3 20 \u5206\u949f\u5de6\u53f3\uff08 2000-3000 \u8f6c \/ \u5206\uff09\u3002\u4ed4\u7ec6\u6536\u96c6\u4e0a\u6e05\u3002\u5982\u6709\u6c89\u6dc0\u5f62 \u6210\uff0c\u5e94\u518d\u6b21\u79bb\u5fc3\u3002
\n\u25c7<\/strong>\u00a0<\/strong>\u5c3f\u6db2\u3001\u80f8\u8179\u6c34\u3001\u8111\u810a\u6db2\uff1a<\/strong>\u00a0<\/strong>
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\n\u25c7<\/strong>\u00a0<\/strong>\u7ec6\u80de\u57f9\u517b\u4e0a\u6e05\uff1a<\/strong>\u00a0<\/strong>
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\n\u25c7<\/strong>\u00a0<\/strong>\u7ec4\u7ec7\u6807\u672c\uff1a<\/strong>\u00a0<\/strong>
\n\u5207\u5272\u6807\u672c\u540e\uff0c\u79f0\u53d6\u91cd\u91cf\u3002\u52a0\u5165\u4e00\u5b9a\u91cf\u7684 PBS \uff0c\u7f13\u51b2\u6db2\u4e2d\u53ef\u52a0\u5165 1 \u03bc g\/L \u86cb\u767d\u9176\u6291\u5236\u5242\u6216 50U\/ml \u7684 Aprotinin \uff08 \u6291\u80bd\u9176\uff09\u3002\u7528\u624b\u5de5\u6216\u5300\u6d46\u5668\u5c06\u6807\u672c\u5300\u6d46\u5145\u5206\u3002\u79bb\u5fc3 20 \u5206\u949f\u5de6\u53f3\uff08 2000-3000 \u8f6c \/ \u5206\uff09\u3002\u4ed4\u7ec6\u6536\u96c6\u4e0a\u6e05\u7f6e\u4e8e -20 \u5ea6\u6216 – 70 \u5ea6\u4fdd\u5b58\uff0c\u5982\u6709\u5fc5\u8981\uff0c\u53ef\u4ee5\u5c06\u6837\u54c1\u6d53\u7f29\u5e72\u71e5\u3002\u5206\u88c5\u540e\u4e00\u4efd\u5f85\u68c0\u6d4b\uff0c\u5176\u4f59\u51b7\u51bb\u5907\u7528\u3002
\n\u5bc4\u6807\u672c\u65f6\u9700\u6ce8\u660e\u4ee5\u4e0b\u60c5\u51b5\uff1a<\/strong>\u00a0<\/strong><\/p>\n

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